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Image Search Results
Journal: Redox Biology
Article Title: Melatonin protects against environmental stress-induced fetal growth restriction via suppressing ROS-mediated GCN2/ATF4/BNIP3-dependent mitophagy in placental trophoblasts
doi: 10.1016/j.redox.2021.101854
Figure Lengend Snippet: Melatonin (MT) alleviates environmental stress-induced progesterone synthesis suppression in placental trophoblasts. (A–F) Pregnant mice in Cd and MT + Cd groups received intraperitoneal injection of Cd on GD8. In MT and MT + Cd groups, pregnant mice were treated with MT from GD7 to GD15. Pregnant mice in Ctrl group were treated with saline. n = 10–12 from 10 to 12 different pregnant mice. (A) Progesterone (P4) in maternal sera. (B) P4 in placentae. (C) P4 in amniotic fluid. (D) Representative immunoblots of CYP11A1 protein in placentae. (E) Quantification for CYP11A1. (F-H) Human JEG-3 cells were pretreated with MT before Cd stimulation (n = 3 per group). (F) P4 in cell medium. (G) Representative immunoblots of CYP11A1 protein in the cells. (H) Quantification for CYP11A1. Data are expressed as the mean ± SEM . Multiple comparisons were performed using ANOVA. The post hoc test is executed adopting Bonferroni or Tamhane's T2 method following the result of homogeneity of variance test. * P < 0.05, ** P < 0.01 versus PBS/NS group; # P < 0.05, ## P < 0.01 versus Cd group.
Article Snippet:
Techniques: Injection, Saline, Western Blot